Increased specificity of reverse transcription priming by trehalose and oligo-blockers allows high-efficiency window separation of mRNA display.

We have developed a method for high-efficiency window separation of cDNA display by increasing the specificity of priming in reverse transcription. In the conventional method, two-base anchored oligo(dT) primers (5'dT16VN3', where N is any base and V is G, A or C) are used to make windows for the di...

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Опубликовано в::	Nucleic Acids Res
Главные авторы:	Mizuno, Y, Carninci, P, Okazaki, Y, Tateno, M, Kawai, J, Amanuma, H, Muramatsu, M, Hayashizaki, Y
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Oxford University Press 1999
Предметы:	Research Article
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148322/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9973624/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.5.1345
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