Gene replacement with linear DNA in electroporated wild-type Escherichia coli.
Gene replacement using linear double-stranded DNA fragments in wild-type Escherichia coli transformation is generally inefficient due to exonucleolytic degradation of incoming DNA. Recombination-proficient strains, in which the exonucleolytic activity of RecBCD is inactivated, have been used as tran...
保存先:
| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1999
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148315/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9973617/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.5.1296 |
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