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Insertion of foreign DNA into plasmids from gram-positive bacteria induces formation of high-molecular-weight plasmid multimers.

Plasmids pUB110, pC194, pE194, and pT181 are commonly used as cloning vectors in both Bacillus subtilis and Staphylococcus aureus. We report that insertion of foreign DNA into any of these plasmids results in the generation of high-molecular-weight plasmid multimers (HMW) of the recombinant, present...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Gruss, A, Ehrlich, S D
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210890/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3125152/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.3.1183-1190.1988
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