Gene replacement with linear DNA in electroporated wild-type Escherichia coli.

Gene replacement using linear double-stranded DNA fragments in wild-type Escherichia coli transformation is generally inefficient due to exonucleolytic degradation of incoming DNA. Recombination-proficient strains, in which the exonucleolytic activity of RecBCD is inactivated, have been used as tran...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	El Karoui, M, Amundsen, S K, Dabert, P, Gruss, A
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 1999
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148315/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9973617/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.5.1296
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