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An allylic/acyclic adenosine nucleoside triphosphate for termination of DNA synthesis by DNA template-dependent polymerases.
An allylic adenosine triphosphate analog (AATP) was tested as a substrate for commercially available DNA polymerases. All but one of the enzymes assayed incorporated AATP opposite thymidine (T) with concomitant termination of the elongation reaction. A concentration of only 1 microM was sufficient f...
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Main Authors: | , , , |
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Format: | Artigo |
Sprog: | Inglês |
Udgivet: |
1999
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Fag: | |
Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC148311/ https://ncbi.nlm.nih.gov/pubmed/9973613 |
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