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Role of Conserved Amino Acids in the Catalytic Activity of Escherichia coli Primase

The role of conserved amino acid residues in the polymerase domain of Escherichia coli primase has been studied by mutagenesis. We demonstrate that each of the conserved amino acids Arg146, Arg221, Tyr230, Gly266, and Asp311 is involved in the process of catalysis. Residues Glu265 and Asp309 are als...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Rodina, Anna, Godson, G. Nigel
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2006
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1482863/
https://ncbi.nlm.nih.gov/pubmed/16672615
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.188.10.3614-3621.2006
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