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Role of Conserved Amino Acids in the Catalytic Activity of Escherichia coli Primase

The role of conserved amino acid residues in the polymerase domain of Escherichia coli primase has been studied by mutagenesis. We demonstrate that each of the conserved amino acids Arg146, Arg221, Tyr230, Gly266, and Asp311 is involved in the process of catalysis. Residues Glu265 and Asp309 are als...

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Main Authors: Rodina, Anna, Godson, G. Nigel
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2006
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1482863/
https://ncbi.nlm.nih.gov/pubmed/16672615
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.188.10.3614-3621.2006
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