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Role of Conserved Amino Acids in the Catalytic Activity of Escherichia coli Primase
The role of conserved amino acid residues in the polymerase domain of Escherichia coli primase has been studied by mutagenesis. We demonstrate that each of the conserved amino acids Arg146, Arg221, Tyr230, Gly266, and Asp311 is involved in the process of catalysis. Residues Glu265 and Asp309 are als...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2006
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1482863/ https://ncbi.nlm.nih.gov/pubmed/16672615 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.188.10.3614-3621.2006 |
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