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Isolation of the Cdc45/Mcm2–7/GINS (CMG) complex, a candidate for the eukaryotic DNA replication fork helicase

The protein Cdc45 plays a critical but poorly understood role in the initiation and elongation stages of eukaryotic DNA replication. To study Cdc45's function in DNA replication, we purified Cdc45 protein from Drosophila embryo extracts by a combination of traditional and immunoaffinity chromat...

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Autors principals: Moyer, Stephen E., Lewis, Peter W., Botchan, Michael R.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2006
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1482467/
https://ncbi.nlm.nih.gov/pubmed/16798881
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0602400103
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