A single cleavage assay for T5 5'-->3' exonuclease: determination of the catalytic parameters forwild-type and mutant proteins.

Bacteriophage T5 5'-->3' exonuclease is a member of a family of sequence related 5'-nucleases which play an essential role in DNA replication. The 5'-nucleases have both exonucleolytic and structure-specific endo-nucleolytic DNA cleavage activity and are conserved in organisms...

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Bibliographic Details
Main Authors: Pickering, T J, Garforth, S J, Thorpe, S J, Sayers, J R, Grasby, J A
Format: Artigo
Language:Inglês
Published: 1999
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC148240/
https://ncbi.nlm.nih.gov/pubmed/9889266
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