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A single cleavage assay for T5 5'-->3' exonuclease: determination of the catalytic parameters forwild-type and mutant proteins.

Bacteriophage T5 5'-->3' exonuclease is a member of a family of sequence related 5'-nucleases which play an essential role in DNA replication. The 5'-nucleases have both exonucleolytic and structure-specific endo-nucleolytic DNA cleavage activity and are conserved in organisms as diverse as bacterio...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Principais autores: Pickering, T J, Garforth, S J, Thorpe, S J, Sayers, J R, Grasby, J A
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1999
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148240/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9889266/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.3.730
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