A detailed study of the substrate specificity of a chimeric restriction enzyme.
Recently, the crystal structure of the designed zinc finger protein, DeltaQNK, bound to a preferred DNA sequence was reported. We have converted DeltaQNK into a novel site-specific endonuclease by linking it to the Fok I cleavage domain (FN). The substrate specificity and DNA cleavage properties of...
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| 發表在: | Nucleic Acids Res |
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| Principais autores: | , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
1999
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148231/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9862996/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.2.674 |
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