A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells.

We have developed an episomal replicating expression vector in which the SV40 gene coding for the large T-antigen was replaced by chromosomal scaffold/matrix attached regions. Southern analysis as well as vector rescue experiments in CHO cells and in Escherichia coli demonstrate that the vector repl...

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Detaylı Bibliyografya
Asıl Yazarlar: Piechaczek, C, Fetzer, C, Baiker, A, Bode, J, Lipps, H J
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1999
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC148196/
https://ncbi.nlm.nih.gov/pubmed/9862961
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