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A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells.

We have developed an episomal replicating expression vector in which the SV40 gene coding for the large T-antigen was replaced by chromosomal scaffold/matrix attached regions. Southern analysis as well as vector rescue experiments in CHO cells and in Escherichia coli demonstrate that the vector repl...

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Udgivet i:Nucleic Acids Res
Principais autores: Piechaczek, C, Fetzer, C, Baiker, A, Bode, J, Lipps, H J
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1999
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148196/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9862961/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.2.426
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