Cloning and stable maintenance of DNA fragments over 300 kb in Escherichia coli with conventional plasmid-based vectors.
Bacterial artificial chromosome (BAC) and P1-derived artificial chromosome (PAC) systems were previously developed for cloning of very large eukaryotic DNA fragments in bacteria. We report the feasibility of cloning very large fragments of eukaryotic DNA in bacteria using conventional plasmid-based...
Shranjeno v:
| izdano v: | Nucleic Acids Res |
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| Principais autores: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1998
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147936/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9776751/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.21.4901 |
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