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Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as large as 100 kilobase pairs.
The development of a bacteriophage P1 cloning system capable of accepting DNA fragments as large as 100 kilobase pairs (kbp) is described. The vectors used in this system contain a P1 packaging site (pac) to package vector and cloned DNA into phage particles, two P1 loxP recombination sites to cycli...
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1990
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC53208/ https://ncbi.nlm.nih.gov/pubmed/2404272 |
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