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Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as large as 100 kilobase pairs.

The development of a bacteriophage P1 cloning system capable of accepting DNA fragments as large as 100 kilobase pairs (kbp) is described. The vectors used in this system contain a P1 packaging site (pac) to package vector and cloned DNA into phage particles, two P1 loxP recombination sites to cycli...

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Detaylı Bibliyografya
Yayımlandı:Proc Natl Acad Sci U S A
Yazar: Sternberg, N
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: National Academy of Sciences 1990
Konular:
Online Erişim:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53208/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2404272/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.1.103
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