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Cloning and stable maintenance of DNA fragments over 300 kb in Escherichia coli with conventional plasmid-based vectors.

Bacterial artificial chromosome (BAC) and P1-derived artificial chromosome (PAC) systems were previously developed for cloning of very large eukaryotic DNA fragments in bacteria. We report the feasibility of cloning very large fragments of eukaryotic DNA in bacteria using conventional plasmid-based...

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Bibliografische gegevens
Gepubliceerd in:Nucleic Acids Res
Hoofdauteurs: Tao, Q, Zhang, H B
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 1998
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147936/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9776751/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.21.4901
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