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Urea improves efficiency of bisulphite-mediated sequencing of 5'-methylcytosine in genomic DNA.

The detection of 5'-methylcytosine by the bisulphite-mediated genomic sequencing method has considerably aided study of the role of methylation in areas such as X chromosome inactivation, genomic imprinting and cancer research. However on occasion difficulty has been experienced in obtaining co...

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Bibliografske podrobnosti
Main Authors: Paulin, R, Grigg, G W, Davey, M W, Piper, A A
Format: Artigo
Jezik:Inglês
Izdano: 1998
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC147923/
https://ncbi.nlm.nih.gov/pubmed/9776768
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