Urea improves efficiency of bisulphite-mediated sequencing of 5'-methylcytosine in genomic DNA.

The detection of 5'-methylcytosine by the bisulphite-mediated genomic sequencing method has considerably aided study of the role of methylation in areas such as X chromosome inactivation, genomic imprinting and cancer research. However on occasion difficulty has been experienced in obtaining complet...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Paulin, R, Grigg, G W, Davey, M W, Piper, A A
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1998
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147923/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9776768/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.21.5009
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