A method for global protein expression and antibody screening on high-density filters of an arrayed cDNA library.
We have developed a technique to establish catalogues of protein products of arrayed cDNA clones identified by DNA hybridisation or sequencing. A human fetal brain cDNA library was directionally cloned in a bacterial vector that allows IPTG-inducible expression of His6-tagged fusion proteins. Using...
Zapisane w:
| Wydane w: | Nucleic Acids Res |
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| Główni autorzy: | , , , , , , |
| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
Oxford University Press
1998
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147919/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9776767/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.21.5007 |
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