A method for global protein expression and antibody screening on high-density filters of an arrayed cDNA library.

We have developed a technique to establish catalogues of protein products of arrayed cDNA clones identified by DNA hybridisation or sequencing. A human fetal brain cDNA library was directionally cloned in a bacterial vector that allows IPTG-inducible expression of His6-tagged fusion proteins. Using...

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Dades bibliogràfiques

Publicat a:	Nucleic Acids Res
Autors principals:	Büssow, K, Cahill, D, Nietfeld, W, Bancroft, D, Scherzinger, E, Lehrach, H, Walter, G
Format:	Artigo
Idioma:	Inglês
Publicat:	Oxford University Press 1998
Matèries:	Research Article
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147919/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9776767/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.21.5007
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