A method for global protein expression and antibody screening on high-density filters of an arrayed cDNA library.

We have developed a technique to establish catalogues of protein products of arrayed cDNA clones identified by DNA hybridisation or sequencing. A human fetal brain cDNA library was directionally cloned in a bacterial vector that allows IPTG-inducible expression of His6-tagged fusion proteins. Using...

Descrizione completa

Dettagli Bibliografici

Pubblicato in:	Nucleic Acids Res
Autori principali:	Büssow, K, Cahill, D, Nietfeld, W, Bancroft, D, Scherzinger, E, Lehrach, H, Walter, G
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1998
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147919/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9776767/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.21.5007
Tags:	Aggiungi Tag Nessun Tag, puoi essere il primo ad aggiungerne!!