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Molecular beacon probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA.

Molecular beacon probes can be employed in a NASBA amplicon detection system to generate a specific fluorescent signal concomitantly with amplification. A molecular beacon, designed to hybridize within the target sequence, was introduced into NASBA reactions that amplify the genomic RNA of potato le...

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Autors principals: Leone, G, van Schijndel, H, van Gemen, B, Kramer, F R, Schoen, C D
Format: Artigo
Idioma:Inglês
Publicat: 1998
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC147533/
https://ncbi.nlm.nih.gov/pubmed/9547273
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