Principles of quantitation of viral loads using nucleic acid sequence-based amplification in combination with homogeneous detection using molecular beacons

For quantitative NASBA-based viral load assays using homogeneous detection with molecular beacons, such as the NucliSens EasyQ HIV-1 assay, a quantitation algorithm is required. During the amplification process there is a constant growth in the concentration of amplicons to which the beacon can bind...

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Main Authors: Weusten, Jos J. A. M., Carpay, Wim M., Oosterlaken, Tom A. M., van Zuijlen, Martien C. A., van de Wiel, Paul A.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2002
Assuntos:
NAR Methods Online
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC101370/
https://ncbi.nlm.nih.gov/pubmed/11884645
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