Principles of quantitation of viral loads using nucleic acid sequence-based amplification in combination with homogeneous detection using molecular beacons

For quantitative NASBA-based viral load assays using homogeneous detection with molecular beacons, such as the NucliSens EasyQ HIV-1 assay, a quantitation algorithm is required. During the amplification process there is a constant growth in the concentration of amplicons to which the beacon can bind...

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Publicado en:	Nucleic Acids Res
Principais autores:	Weusten, Jos J. A. M., Carpay, Wim M., Oosterlaken, Tom A. M., van Zuijlen, Martien C. A., van de Wiel, Paul A.
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 2002
Assuntos:	NAR Methods Online
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC101370/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11884645/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/30.6.e26
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