Simple and efficient generation in vitro of nested deletions and inversions: Tn5 intramolecular transposition.
We have exploited the intramolecular transposition preference of the Tn 5 in vitro transposition system to test its effectiveness as a tool for generation of nested families of deletions and inversions. A synthetic transposon was constructed containing an ori, an ampicillin resistance (Ampr) gene, a...
Sparad:
| I publikationen: | Nucleic Acids Res |
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| Huvudupphov: | , , , |
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Utgiven: |
Oxford University Press
1998
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| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147497/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9518484/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.8.1927 |
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