Simple and efficient generation in vitro of nested deletions and inversions: Tn5 intramolecular transposition.

We have exploited the intramolecular transposition preference of the Tn 5 in vitro transposition system to test its effectiveness as a tool for generation of nested families of deletions and inversions. A synthetic transposon was constructed containing an ori, an ampicillin resistance (Ampr) gene, a...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	York, D, Welch, K, Goryshin, I Y, Reznikoff, W S
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1998
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147497/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9518484/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.8.1927
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