Simple and efficient generation in vitro of nested deletions and inversions: Tn5 intramolecular transposition.

We have exploited the intramolecular transposition preference of the Tn 5 in vitro transposition system to test its effectiveness as a tool for generation of nested families of deletions and inversions. A synthetic transposon was constructed containing an ori, an ampicillin resistance (Ampr) gene, a...

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Sparad:
Bibliografiska uppgifter
Huvudupphovsmän: York, D, Welch, K, Goryshin, I Y, Reznikoff, W S
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1998
Ämnen:
Research Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC147497/
https://ncbi.nlm.nih.gov/pubmed/9518484
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