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Detection of mutations in GC-rich DNA by bisulphite denaturing gradient gel electrophoresis.

Denaturing gradient gel electrophoresis (DGGE) in combination with PCR and 'GC-clamping' has proven highly efficient as a method for detection of DNA sequence differences. Due to strand dissociation phenomena, however, its use has been limited to the analysis of sequences with a relatively low conte...

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Publicado en:Nucleic Acids Res
Principais autores: Guldberg, P, Grønbak, K, Aggerholm, A, Platz, A, thor Straten, P, Ahrenkiel, V, Hokland, P, Zeuthen, J
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 1998
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147432/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9490806/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.6.1548
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