Application of Denaturing Gradient Gel Electrophoresis (DGGE) To Study the Diversity of Marine Picoeukaryotic Assemblages and Comparison of DGGE with Other Molecular Techniques

We used denaturing gradient gel electrophoresis (DGGE) to study the diversity of picoeukaryotes in natural marine assemblages. Two eukaryote-specific primer sets targeting different regions of the 18S rRNA gene were tested. Both primer sets gave a single band when used with algal cultures and comple...

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Détails bibliographiques
Auteurs principaux: Díez, Beatriz, Pedrós-Alió, Carlos, Marsh, Terence L., Massana, Ramon
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2001
Sujets:
Microbial Ecology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC92965/
https://ncbi.nlm.nih.gov/pubmed/11425706
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.7.2942-2951.2001
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