High-Efficiency Transfection of Primary Human and Mouse T Lymphocytes Using RNA Electroporation

The use of nonviral gene transfer methods in primary lymphocytes has been hampered by low gene transfer efficiency and high transfection-related toxicity. In this report, high gene transfection efficiency with low transfection-related toxicity was achieved by electroporation using in vitro-transcrib...

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Main Authors: Zhao, Yangbing, Zheng, Zhili, Cohen, Cyrille J., Gattinoni, Luca, Palmer, Douglas C., Restifo, Nicholas P., Rosenberg, Steven A., Morgan, Richard A.
Format: Artigo
Jezik:Inglês
Izdano: 2005
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1473967/
https://ncbi.nlm.nih.gov/pubmed/16140584
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymthe.2005.07.688
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