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Direct cloning of DNA that interacts in vivo with a specific protein: application to RNA polymerase II and sites of pausing in Drosophila.

A new method is described for cloning DNA sequences occupied by a specific protein on chromatin in vivo . The approach uses UV cross-linking to couple proteins covalently to DNA and the resulting complexes are then purified under stringent conditions. Particular adducts are immunoprocipitated with a...

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Kaydedildi:
Detaylı Bibliyografya
Yayımlandı:Nucleic Acids Res
Asıl Yazarlar: Law, A, Hirayoshi, K, O'Brien, T, Lis, J T
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Oxford University Press 1998
Konular:
Online Erişim:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147354/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9461448/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.4.919
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