Direct cloning of DNA that interacts in vivo with a specific protein: application to RNA polymerase II and sites of pausing in Drosophila.
A new method is described for cloning DNA sequences occupied by a specific protein on chromatin in vivo . The approach uses UV cross-linking to couple proteins covalently to DNA and the resulting complexes are then purified under stringent conditions. Particular adducts are immunoprocipitated with a...
Spremljeno u:
| Izdano u: | Nucleic Acids Res |
|---|---|
| Glavni autori: | , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1998
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147354/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9461448/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.4.919 |
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