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Direct cloning of DNA that interacts in vivo with a specific protein: application to RNA polymerase II and sites of pausing in Drosophila.

A new method is described for cloning DNA sequences occupied by a specific protein on chromatin in vivo . The approach uses UV cross-linking to couple proteins covalently to DNA and the resulting complexes are then purified under stringent conditions. Particular adducts are immunoprocipitated with a...

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Udgivet i:Nucleic Acids Res
Principais autores: Law, A, Hirayoshi, K, O'Brien, T, Lis, J T
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1998
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147354/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9461448/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.4.919
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