Interaction and effect of annealing temperature on primers used in differential display RT-PCR.

Differential display of mRNA is a simple, sensitive and powerful method to identify differentially expressed gene fragments. The main drawback of differential display is the lack of reproducibility and the inability to read and compare complex gels. This issue results from employing unoptimized prim...

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Detalles Bibliográficos

Publicado en:	Nucleic Acids Res
Principais autores:	Malhotra, K, Foltz, L, Mahoney, W C, Schueler, P A
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 1998
Assuntos:	Research Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147309/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9443980/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.3.854
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