Interaction and effect of annealing temperature on primers used in differential display RT-PCR.

Differential display of mRNA is a simple, sensitive and powerful method to identify differentially expressed gene fragments. The main drawback of differential display is the lack of reproducibility and the inability to read and compare complex gels. This issue results from employing unoptimized prim...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Malhotra, K, Foltz, L, Mahoney, W C, Schueler, P A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1998
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147309/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9443980/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.3.854
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