Interaction and effect of annealing temperature on primers used in differential display RT-PCR.

Differential display of mRNA is a simple, sensitive and powerful method to identify differentially expressed gene fragments. The main drawback of differential display is the lack of reproducibility and the inability to read and compare complex gels. This issue results from employing unoptimized prim...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Malhotra, K, Foltz, L, Mahoney, W C, Schueler, P A
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1998
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147309/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9443980/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.3.854
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