Specific polyadenylation and purification of total messenger RNA from Escherichia coli.
Obtaining pure mRNA preparations from prokaryotes has been difficult, if not impossible, for want of a poly(A) tail on these messages. We have used poly(A) polymerase from yeast to effect specific polyadenylation of Escherichia coli polysomal mRNA in the presence of magnesium and manganese. The poly...
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| Publicado en: | Nucleic Acids Res |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
1997
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146910/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9254705/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.17.3465 |
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