Specific polyadenylation and purification of total messenger RNA from Escherichia coli.

Obtaining pure mRNA preparations from prokaryotes has been difficult, if not impossible, for want of a poly(A) tail on these messages. We have used poly(A) polymerase from yeast to effect specific polyadenylation of Escherichia coli polysomal mRNA in the presence of magnesium and manganese. The poly...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Amara, R R, Vijaya, S
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1997
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146910/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9254705/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.17.3465
Tags:	Tag hinzufügen Keine Tags, Fügen Sie das erste Tag hinzu!