Specific polyadenylation and purification of total messenger RNA from Escherichia coli.
Obtaining pure mRNA preparations from prokaryotes has been difficult, if not impossible, for want of a poly(A) tail on these messages. We have used poly(A) polymerase from yeast to effect specific polyadenylation of Escherichia coli polysomal mRNA in the presence of magnesium and manganese. The poly...
Αποθηκεύτηκε σε:
| Εκδόθηκε σε: | Nucleic Acids Res |
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| Κύριοι συγγραφείς: | , |
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
Oxford University Press
1997
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| Θέματα: | |
| Διαθέσιμο Online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146910/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9254705/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.17.3465 |
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