Specific polyadenylation and purification of total messenger RNA from Escherichia coli.

Obtaining pure mRNA preparations from prokaryotes has been difficult, if not impossible, for want of a poly(A) tail on these messages. We have used poly(A) polymerase from yeast to effect specific polyadenylation of Escherichia coli polysomal mRNA in the presence of magnesium and manganese. The poly...

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Argitaratua izan da:	Nucleic Acids Res
Egile Nagusiak:	Amara, R R, Vijaya, S
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	Oxford University Press 1997
Gaiak:	Research Article
Sarrera elektronikoa:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146910/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9254705/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.17.3465
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