Brief expression of a GFP cre fusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletions.
The Cre DNA recombinase of bacteriophage P1 has become a useful tool for precise genomic manipulation in embryonic stem (ES) cells that have been gene modified by homologous recombination. We have re-engineered the cre gene to allow ready identification of living Cre+cells by constructing a function...
Tallennettuna:
| Julkaisussa: | Nucleic Acids Res |
|---|---|
| Päätekijät: | , , , |
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
Oxford University Press
1997
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146882/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9241248/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.16.3326 |
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