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Brief expression of a GFP cre fusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletions.

The Cre DNA recombinase of bacteriophage P1 has become a useful tool for precise genomic manipulation in embryonic stem (ES) cells that have been gene modified by homologous recombination. We have re-engineered the cre gene to allow ready identification of living Cre+cells by constructing a function...

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Dades bibliogràfiques
Publicat a:Nucleic Acids Res
Autors principals: Gagneten, S, Le, Y, Miller, J, Sauer, B
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1997
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146882/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9241248/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.16.3326
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