DNA sequencing using differential extension with nucleotide subsets (DENS).

Here we describe template directed enzymatic synthesis of unique primers, avoiding the chemical synthesis step in primer walking. We have termed this conceptually new technique DENS (differential extension with nucleotide subsets). DENS works by selectively extending a short primer, making it a long...

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Autors principals: Raja, M C, Zevin-Sonkin, D, Shwartzburd, J, Rozovskaya, T A, Sobolev, I A, Chertkov, O, Ramanathan, V, Lvovsky, L, Ulanovsky, L E
Format: Artigo
Idioma:Inglês
Publicat: 1997
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC146503/
https://ncbi.nlm.nih.gov/pubmed/9016632
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