DNA sequencing using differential extension with nucleotide subsets (DENS).

Here we describe template directed enzymatic synthesis of unique primers, avoiding the chemical synthesis step in primer walking. We have termed this conceptually new technique DENS (differential extension with nucleotide subsets). DENS works by selectively extending a short primer, making it a long...

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Detalhes bibliográficos
Main Authors: Raja, M C, Zevin-Sonkin, D, Shwartzburd, J, Rozovskaya, T A, Sobolev, I A, Chertkov, O, Ramanathan, V, Lvovsky, L, Ulanovsky, L E
Formato: Artigo
Idioma:Inglês
Publicado em: 1997
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC146503/
https://ncbi.nlm.nih.gov/pubmed/9016632
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