High efficiency of site-directed mutagenesis mediated by a single PCR product.

We describe a highly efficient procedure for site-specific mutagenesis of double-stranded plasmids. The method relies on a single PCR primer which incorporates both the mutations at the selection site and the desired single base substitutions at the mutant site. This primer is annealed to the denatu...

Fuld beskrivelse

Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Chen, X, Liu, W, Quinto, I, Scala, G
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 1997
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146466/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9016615/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.3.682
Tags:	Tilføj Tag Ingen Tags, Vær først til at tagge denne postø!