High efficiency of site-directed mutagenesis mediated by a single PCR product.
We describe a highly efficient procedure for site-specific mutagenesis of double-stranded plasmids. The method relies on a single PCR primer which incorporates both the mutations at the selection site and the desired single base substitutions at the mutant site. This primer is annealed to the denatu...
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| Publié dans: | Nucleic Acids Res |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
1997
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146466/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9016615/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.3.682 |
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