An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria.

A high yield, rapid and simple procedure is described for extracting RNA from mycobacteria and other micro-organisms refractory to disruption. The method yielded 20 microg RNA/109 Mycobacterium bovis BCG, more than 10 times greater than our previous method. Intact full length hsp 70 (dnaK) mRNA was...

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Main Authors: Mangan, J A, Sole, K M, Mitchison, D A, Butcher, P D
Format: Artigo
Sprog:Inglês
Udgivet: 1997
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC146465/
https://ncbi.nlm.nih.gov/pubmed/9016612
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