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An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria.
A high yield, rapid and simple procedure is described for extracting RNA from mycobacteria and other micro-organisms refractory to disruption. The method yielded 20 microg RNA/109 Mycobacterium bovis BCG, more than 10 times greater than our previous method. Intact full length hsp 70 (dnaK) mRNA was...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1997
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC146465/ https://ncbi.nlm.nih.gov/pubmed/9016612 |
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