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Comparison of Methods of Extracting Intracellular Proteases from Bacteria

Five commonly used methods of disintegrating bacterial cells were compared by use of Bacillus subtilis, Pseudomonas putrefaciens, and Streptococcus durans as the test organisms. These methods were: (i) sonic treatment, (ii) grinding, (iii) freezing and thawing, (iv) acetone-powder, and (v) toluene....

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Autors principals: Vadehra, D. V., Wallace, D. L., Harmon, L. G.
Format: Artigo
Idioma:Inglês
Publicat: 1965
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1058387/
https://ncbi.nlm.nih.gov/pubmed/4956035
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