Recombination-mediated PCR-directed plasmid construction in vivo in yeast.

We have extended the technique of PCR-directed recombination in Saccharomyces cerevisiae to develop a simple method for plasmid or gene construction in the absence of suitable restriction sites. The DNA to be cloned is PCR-amplified with 30-40 bp of homology to a linearized yeast plasmid. Co-transfo...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Oldenburg, K R, Vo, K T, Michaelis, S, Paddon, C
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146432/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9016579/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.2.451
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