Recombination-mediated PCR-directed plasmid construction in vivo in yeast.
We have extended the technique of PCR-directed recombination in Saccharomyces cerevisiae to develop a simple method for plasmid or gene construction in the absence of suitable restriction sites. The DNA to be cloned is PCR-amplified with 30-40 bp of homology to a linearized yeast plasmid. Co-transfo...
Uloženo v:
| Vydáno v: | Nucleic Acids Res |
|---|---|
| Hlavní autoři: | , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
1997
|
| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146432/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9016579/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.2.451 |
| Tagy: |
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
|